Extended Data Fig. 3: Genomically encoded lpxM accurately predicts lipid A hexa-acylation capacity of gut microbes.
From: Gut microbiota-derived hexa-acylated lipopolysaccharides enhance cancer immunotherapy responses
Two commensal isolates from our culture collection that encode lpxM were grown anaerobically, and a, lipid A was isolated from resulting cell pellets and analyzed using a customized LC-MS lipidomics method for characterization of lipid A acylation. b, MS/MS spectra of one characteristic hexa-acyl-1P ion (m/z 1716) of a purchased standard lipid A-1P from Escherichia coli. The characteristic acyl chain losses are annotated in the spectra as described by Sándor et al., 2016. c, Overlaid extracted ion chromatogram (EIC) of lipid A from mouse E. coli isolate LPS (left) and relative composition in terms of acylation and phosphorylation (right). d, Overlaid EIC of lipid A from mouse Klebsiella pneumoniae isolate LPS (left) and relative composition in terms of acylation and phosphorylation (right).
