Extended Data Fig. 8: Proteins and crystal analysis.
From: Structural insights into tecovirimat antiviral activity and poxvirus resistance
a) SDS-PAGE of the proteins used in this manuscript. Molecular weight markers are shown in the left lane. In the other lanes, 1 μg of sF13 wild-type and mutants have been loaded, as indicated. For the sake of clarity, we have prepared a gel with all proteins at the same time, side by side. All of them have been analyzed at least 3 times on SDS-PAGE with identical results. b) Crystal structure of the sF13/tecovirimat complex processed in different space groups, as indicated. 2Fo-Fc maps contoured at 1σ are shown in blue, Fo-Fc maps contoured at +3σ and -3σ are shown in green and red, respectively. In the cubic space group (F432), a 2-fold symmetry axis passes through the center of the tecovirimat molecule, so the density represents two tecovirimat molecules with 50% occupancy each. Only one of the molecules is shown. In the central panel, we display the electron density resulting from refining the structure in P1, using a single orientation for tecovirimat (100% occupancy). Similarly, in the right panel, we process the data in P1 but refine the structure using two orientations with 50% occupancy each.
