Extended Data Fig. 5: Epigenetic characteristics observed in proviral regions infected with wild-type (wt) or silencer-mutated (s-mut) HTLV-1.
From: Intragenic viral silencer element regulates HTLV-1 latency via RUNX complex recruitment
a, Quantification of cell-associated HTLV-1, measured by tax as total HTLV-1 and 2-LTR circle as episomal HTLV-1 DNA in JET cells infected with wt- or s-mut- HTLV-1. b, ChIP-quantitative PCR (qPCR) results for H3K4me3 and H3K9Ac of HTLV-1 infected JET cells in the HTLV-1 provirus. c, Schematic representation of Chromatin Conformation Capture (3 C) to evaluate HTLV-1 LTR-OCR proximity. d, Quantification of LTR-OCR proximity in JET cells infected with wt- or s-mut- HTLV-1. A representative data from 2 independent experiments is shown. Bars and error bars represent the mean ± SD of results in triplicate experiments. p value was calculated using a two-sided, unpaired Student’s t-test.
