Extended Data Fig. 4: Cell type-specificity and molecular mechanisms underlying the OCR function.
From: Intragenic viral silencer element regulates HTLV-1 latency via RUNX complex recruitment
a-b, The OCR showed suppressive effect on the 5′-LTR in T cells but not in non-T cell lines. Non-T and T cell lines (a) human primary CD4 + T cells and iPS-ML cells (b) were used for the luciferase assays at 48 hours after transfection. c-d, Effect of the shRNA targeting RUNX1 on RUNX1 expression in Molt4 cells (c) and Jurkat T cells (d). RUNX1 protein levels were analyzed by flow cytometry analysis with anti-RUNX1 mAb. e, Effect of RUNX1 knock down on the OCR function. f, OCR mediated silencing of 5′-LTR by RUNX1 mutants (P187R and F262V) reported in ATL patients. 293 T cells were used for luciferase reporter assay. At least 2 independent experiments were performed. Bars and error bars represent the mean ± SD of results in triplicate experiments. p values were calculated using a two-sided, unpaired Student’s t-test.
