Extended Data Fig. 5: Impacts of vBcl-2 on mitochondrial function and integrity.
a, Representative confocal micrographs (left) and quantification (right) of PINK1 (magenta) colocalization with TOM20 (green) in iSLK-BAC16.vBcl-2 KO cells expressing mCherry-vBcl-2 (red) at 60 h post-reactivation (n = 100 cells/sample, 3 independent experiments). Scale bars, 10 μm. b, Co-IP of vBcl-2 and endogenous NM23-H2 with DRP1 in HEK293T cells expressing NM23-H2 shRNA or Ctrl shRNA and transduced with Vec or HA-vBcl-2. c, Co-IP of indicated proteins in HEK293T expressing mCherry-vBcl-2 (WT/E14A). The relative amount of HA-NM23-H2 co-immunoprecipitated with DRP1 was normalized to total HA-NM23-H2 in WCL (right; n = 3 independent experiments). d, DRP1 GTPase activity in the presence/absence of purified NM23-H2, vBcl-2 (WT/E14A), and indicated nucleotides (n = 3 independent experiments). Data in a-c are representative of three independent experiments. See Source Data for uncropped data of b,c. Data in a,c,d are mean ± s.d. analyzed by one-way ANOVA with Tukey’s post hoc test. Exact P values are provided in Source Data Extended Data Fig. 5.
