Extended Data Fig. 8: FlgK mutant variants are secreted in comparable amounts to WT FlgK and flagella of FlgKL interface mutants are more prone to break. | Nature Microbiology

Extended Data Fig. 8: FlgK mutant variants are secreted in comparable amounts to WT FlgK and flagella of FlgKL interface mutants are more prone to break.

From: The structure of the complete extracellular bacterial flagellum reveals the mechanism of flagellin incorporation

Extended Data Fig. 8: FlgK mutant variants are secreted in comparable amounts to WT FlgK and flagella of FlgKL interface mutants are more prone to break.

a, Immunoblotting of the cellular and secreted fractions using the pulsed flhDC induction setup of wild-type, a ∆flgKL control strain, the FlgK mutants (D519S and D519R) using anti-FlgK antibody. DnaK serves as a loading and lysis control. One biological replicate performed. b, Quantification of the number of flagella per cell using the pulsed flhDC induction setup in the wild-type and the FlgK D519R and FlgL I44S L260S mutants without shearing and after 20 ×, 40 × or 60 × shearing. Filaments (FliC T237C) were labelled with Dylight555 Maleimide and DNA counterstained with DAPI after pulsed flhDC induction. The number of flagella per cell (ℕ0) was determined for n > 200 individual bacteria per strain and condition for one biological replicate. Violin plots represent the distribution of the data including the median (dashed line) and quartiles (dotted lines).

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