Extended Data Fig. 8: Characterisation of HIV-1 E45A core nuclear import. | Nature Microbiology

Extended Data Fig. 8: Characterisation of HIV-1 E45A core nuclear import.

From: HIV-1 nuclear import is selective and depends on both capsid elasticity and nuclear pore adaptability

Extended Data Fig. 8: Characterisation of HIV-1 E45A core nuclear import.The alternative text for this image may have been generated using AI.

a, Gallery of E45A cores in multiple states during the nuclear import. Six representative tomographic slices from each state are showcased. E45A cores are indicated by purple arrowheads, nuclear factors are indicated by light purple arrowheads, the nucleus, NE (indicated by black dashed lines in some cases), and NPC (indicated by white circles in the top view in some cases) are annotated accordingly. Scale bars = 50 nm. b, A bar chart illustrating the composition of E45A core shapes in each state. Cone-shape cores are in purple and tube-shaped cores are in blue (Two-sided Chi-square test for all, p < 0.0001). c, A bar chart showing the distribution of WT, E45A, and E45A/R132T cores docking at a single NPC. Single: only one core; Multiple: ≥ two cores (Two-sided Chi-square test for all, and two-sided Fisher’s exact test for E45A and WT, p < 0.0001). d, A bar chart showing the orientation distribution of cone-shaped E45A cores in docking and traversing states, with the wide end in first (grey) and narrow end in first (pink) (Two-sided Fisher’s exact test for one comparison, p = 0.0147). e, A violin plot of the statistical analysis on the size of cone-shaped E45A cores (width measured at the wide end) in each state. The size of imported cone-shaped E45A cores measures 50.11 ± 3.180 nm (SE = 1.060, n = 9), the traversing measures 50.86 ± 3.078 nm (SE = 1.164, n = 7), the docking measures 56.86 ± 5.791 nm (SE = 0.3598, n = 259), the approaching measures 56.32 ± 6.054 nm (SE = 0.5172, n = 137), and the input measures 57.89 ± 8.162 nm (SE = 0.5845, n = 195). White lines represent the medians, black lines represent the quartiles, and black dots represent individual cone-shaped E45A cores (One-way ANOVA test for all, ** = p = 0.0059 < 0.01, * = p < 0.05 = 0.0489 for input vs traversing, and = 0.0243 for docking vs imported, only significant differences are shown). f, A violin plot of the statistical analysis on the size of tube-shaped E45A cores (width measured) in each state. The size of imported tube-shaped E45A cores measures 40.45 ± 2.505 nm (SE = 0.7551, n = 11), the traversing measures 42.71 ± 6.921 nm (SE = 2.616, n = 7), the docking measures 42.63 ± 3.596 nm (SE = 0.6921, n = 27), the approaching measures 42.76 ± 4.176 nm (SE = 1.013, n = 17), and the input measures 42.84 ± 3.116 nm (SE = 0.4751, n = 43). White lines represent the medians, black lines represent the quartiles, and black dots represent individual tube-shaped E45A cores (One-way ANOVA test for all, ns = no significance).

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