Fig. 2: STA of HIV-1 WT CA hexamers and NPCs during nuclear import.

a, Structures of CA hexamers in capsid lattices of outside (approaching and docking cores combined), traversing and imported WT cores. Maps are aligned and contoured to the same level. The first column shows top views of coloured CA hexamer density maps (contoured at 3σ); the second column shows top views of CA hexamer density maps (contoured at 3σ) fitted with a CA hexamer model (PDB 6SKK); the third column shows the CA hexamer density maps (contoured at 0.5σ), coloured according to height, from white (bottom) to purple (top); and the fourth column shows the side views of CA hexamer density maps (major body density contoured at 3σ, floating top density contoured at 0.5σ), the distance between CA hexamer and the floating top density measures approximately 63 Å for the imported WT core. b, Structures of NPC ring moieties. The first four columns starting from the left show the structures of NPC CR, IR, LR and NR, respectively. The composite EM map is depicted in the fifth column. The top row depicts the central slice of EM maps in the x–z plane; NPC densities and NE are annotated. The middle and bottom rows show two orthogonal views of EM maps. CR and IR are contoured at 3σ, LR is contoured at 1σ, NR is contoured at 1.5σ and the composite map is contoured at 2σ. Scale bars, 10 nm. c, Composite EM map of the whole CEM NPC with eight subunits. Two orthogonal views of the NPC are depicted.