Extended Data Fig. 7: Library construction for CLIP-seq.
From: Human cytomegalovirus long non-coding RNA counteracts nuclear cGAS to facilitate immune evasion
(a) Immunoblot images representing the IP efficiency of the endogenous cGAS in Fig. 3a (E9G9G; Cell Signaling). (b) Immunoblot images representing the IP efficiency of the cGAS variants in Fig. 3c (F3165; Sigma). (a-b) The representative images of two independent experiments are presented. (c-e) Images were obtained during CLIP-seq library construction. (c) Immunoblot images representing the IP efficiency of cGAS antibodies (D1D3G and E9G9G; Cell signaling). (d) Autoradiograph images of 32P-labeled RNA-cGAS complexes and adaptor-ligated RNAs enriched by cGAS-CLIP. PCR amplicons of CLIP-libraries were visualized by staining with EtBR. (e) Autoradiograph images of 32P-labeled fragmented RNAs from non-crosslinked cells and adaptor-ligated RNAs. PCR amplicons of SMI-libraries were visualized by staining with EtBR. (a-e) See Source Data for uncropped data. (c-e) Two independent experiments are indicated as rep #1 and #2.
