Extended Data Fig. 1: The 1.9–4.9-kb region of RNA4.9 plays a critical role in suppressing innate immune response.
From: Human cytomegalovirus long non-coding RNA counteracts nuclear cGAS to facilitate immune evasion
(a) Graphical abstract describing each recombinant virus. HFFs infected with WT and 1.9–4.9krpsL HCMV (1 MOI, 6 hpi) were analyzed by reverse transcription-qPCR (RT-qPCR) (n = 2). The data represents mean. “n” indicates the number of independent experiments. (b) Top-10 significant GO terms of upregulated DEGs in 1.9–4.9krpsL HCMV infected cells at the indicated time points. The GO term ranking is determined by –log10 (adj.p-value). Adjusted p-values were based on one-sided Fisher’s exact test corrected by Benjamini-Hochberg procedure. Exact adjusted p-values are provided in Supplementray Table 3. (c) IFN-β protein levels in cell-free supernatant of HCMV-infected HFFs (1 MOI) were quantified by enzyme-linked immunosorbent assay (ELISA) at the indicated time points (n = 4). (d) Graphical abstract describing each recombinant virus. (d-e) HFFs infected with WT, 1.9–4.9krpsL, and Δ1.9–4.9k HCMV (1 MOI, 6 hpi) were analyzed by RT-qPCR (n = 3). (c-e) “n” indicates the number of independent experiments. The data are presented as mean ± SEM and P-values are based on two-sided multiple t-tests without correction. Exact P-values are provided in Source Data Extended Data Fig. 1.
