Extended Data Fig. 5: Modification hot spots at 43 °C do not specifically affect tiREN.

Restriction activity following ON growth at 37 °C (blue) or 43 °C (pink) of WT strain and mutants. Mutants are strains lacking the complete ORF containing the modification site. Restriction activity was assessed with un-modified JBD24 phages, relative to the hsdR mutant strain. The data represent the mean of three independent biological replicates +/− SD.