Extended Data Fig. 6: Restriction endonuclease is not transcriptionally regulated. | Nature Microbiology

Extended Data Fig. 6: Restriction endonuclease is not transcriptionally regulated.

From: Multigenerational proteolytic inactivation of restriction upon subtle genomic hypomethylation in Pseudomonas aeruginosa

Extended Data Fig. 6

(a) Global transcriptomics analysis: Samples taken 5 generations into recovery following the 43 °C growth and at the end of the recovery phase (45 generations) were compared to normal growth conditions. Marked dots indicate Hsd proteins: HsdR and HsdMS. (b) Real-time PCR analysis of hsd transcripts following growth at either 37 °C or 43 °C. expression levels are normalized to rpoD as a ctrl. (c) Fluorescence and absorbance measurements over time of a strain harboring the hsdR ORF replaced by mCherry, representing transcriptional reporter analysis. (d) Fluorescence and optical density measurements over time of a strain harboring hsdR fused to mCherry, representing translational reporter analysis. The (c) and (d) graphs represent the mean of three independent biological replicates +/− SD.

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