Extended Data Fig. 2: DNA recombination and breaks do not induce tiREN. | Nature Microbiology

Extended Data Fig. 2: DNA recombination and breaks do not induce tiREN.

From: Multigenerational proteolytic inactivation of restriction upon subtle genomic hypomethylation in Pseudomonas aeruginosa

Extended Data Fig. 2

(a) Restriction activity recombination genes mutant strains at 37 °C (blue) and following 43 °C growth, compared to WT strain. (b) Restriction activity of WT strain following 6 hr growth with sub-inhibitory concentration Fluoroquinolone treatment of Ciprofloxacin and Norfloxacin, compared to untreated WT strain. For the above graphs, restriction activity was assessed by JBD24 non-modified phages infection. For (a) and (b) graphs, the data represent the mean of three independent biological replicates +/− SD.

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