Fig. 1: Formulation and characterization of a 3-phage combination.
a, Host-range map of isolated phages (columns, n = 21) and cocktail-V1 tested against a subset of 36 ECC isolates. Each row represents an ECC isolate, classified on the basis of ST and colour coded accordingly. Singletons are STs that only occurred once. b, Representations of phage genome size and classification of the three phages (øEnA02, øEnC07 and øEnC15) of cocktail-V1. c–e, TEM images of the three phages (øEnA02 (c), øEnC07 (d) and øEnC15 (e)). f–h, One-step growth curve of the three phages (øEnA02 (f), øEnC07 (g) and øEnC15 (h)) in the cocktail propagated on their host of isolation. Data represent mean ± s.e.m of 3 biological replicates. The latency time (in minutes) and burst size (in p.f.u.s per infection) were calculated for each phage. i, Relative EOP values for three phages, represented by different coloured dots (n = 1) across ECC isolates. The upper dotted line represents the EOPs on the host of isolation, which are set as 1. The lower dashed line represents the limit of detection (LOD) of the assay. j, Stability of the three phages (øEnA02, øEnC07 and øEnC15) at 4 °C, room temperature and 37 °C storage conditions. Data represent mean ± s.e.m of 2 biological replicates. M, months.
