Extended Data Fig. 4: Additional eCLIP data, related to Fig. 4.
(a) Enrichment of VSV viral RNA in eCLIP samples from HEK293 cells overexpressing IFIT2-IFIT3. Fraction of viral RNA relative to all RNA (host+viral) from RNAseq (Total) and eCLIP (eCLIP) samples for each indicated VSV infected cell line. Each replicate is shown as a point, with the line showing the average fraction of viral reads. For IFIT2-IFIT3 overexpression, the fold enrichment of IFIT-bound (eCLIP) fraction relative to fraction of viral reads in RNAseq (Total) is indicated. (b) Full VSV genome coverage for additional samples from the eCLIP experiment described in Fig. 4, including uninfected controls, for human IFIT-expressing Flp-In T-REx HEK293 lines. (c) Same as panel a, but for eCLIP samples from VSV infected mouse wild-type and Ifit2 or Ifit3 knockout MEFs. (d) Same as panel b, but for MEFs. (b, d) Values shown are normalized reads per million (RPM) out of all reads mapped to the VSV genome.
