Extended Data Fig. 5: IFIT2-IFIT3 recognizes short viral 5’ UTRs, related to Fig. 5.
(a-b) Plasmids expressing either a (a) long plasmid-derived 5’ UTR or (b) no plasmid-derived 5’ UTR immediately upstream of the VSV-P 5’ UTR were transfected into 293T cells. Twenty-four h post-transfection, cells were harvested, and RNA was isolated for 5’ RACE experiments. Ten clones from each construct were sequenced, and the transcription start site is marked for each plasmid. (c) Experiment was carried out as described in Fig. 5c, but instead of imaging, cells were harvested and lysates were analyzed by western blotting. Densitometry values were calculated using ImageJ. Experiment was performed independently twice. (d) Raw values from normalized data in Fig. 5d-e (n = 4 biological replicates). Statistical analyses: Data are represented as mean ± SEM. (d) Ordinary two-way ANOVA with Šídák’s multiple comparisons test and a single pooled variance. * = p < 0.05, ** = p < 0.01, **** = p < 0.0001.
