Extended Data Fig. 2: Cryo-EM data processing for IFIT2-IFIT3, related to Fig. 2. | Nature Microbiology

Extended Data Fig. 2: Cryo-EM data processing for IFIT2-IFIT3, related to Fig. 2.

From: The IFIT2–IFIT3 antiviral complex targets short 5’ untranslated regions on viral mRNAs for translation inhibition

Extended Data Fig. 2

(a) Workflow for structure determination. See Methods for a detailed explanation of data processing. For 2D classes, classes outlined in red were excluded from later steps. For 3D classes, classes outlined in green were selected for later steps. The final refined map showed a resolution of 3.22 Å (gold-standard FSC criterion of 0.143). (b) Directional Fourier Shell Correlation analysis for the final map, calculated by 3DFSC66. (c) Two views of the final map, colored by local resolution (blue: 2.8 Å or better; red: 3.8 Å or worse).

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