Fig. 3: Root bacterial microbiota composition is more stable in T. dactyloides than Z. mays under varying inocula and water treatments.
From: Precipitation legacy effects on soil microbiota facilitate adaptive drought responses in plants
For a, c, d and f, points are EMMs of biological replicates, error bars represent ±s.e.m., and letters indicate pairwise group differences based on ANOVA followed by a two-tailed Tukey post hoc test and FDR correction (q < 0.05). Units of study were individual plants grown in independent pots. Each sample represents one independent biological replicate. These analyses include n = 146 gamagrass root microbiota samples and n = 264 maize root microbiota samples. a, Shannon diversity (eShannon index) was not significantly affected by precipitation legacy (ANOVA, F1,141 = 0.72, P = 0.39) or test-phase water treatment (ANOVA, F1,143 = 0.04, P = 0.85). b, Constrained ordination by test-phase water treatment and inoculum precipitation legacy (ANOVA-like permutation test, full model, F2,139 = 1.74, R2 = 0.022, P = 0.01) indicated that test-phase water treatment (by term, F1,139 = 2.58, P = 0.003) but not legacy (by term, F1,139 = 0.89, P = 0.55) significantly impacted gamagrass root bacterial composition. Ellipses indicate 95% confidence intervals; solid lines for high precipitation legacy and dashed lines for low precipitation legacy. c, Drought treatment did not significantly affect within-group variation (β-dispersion) of gamagrass root microbiomes (ANOVA, F1,144 = 0.57, P = 0.45). d, Maize root microbiota Shannon diversity was significantly affected by test-phase water treatment (ANOVA, F1,249 = 20.97, P = 0.000007), but not by inoculum precipitation legacy (ANOVA, F1,259 = 1.96, P = 0.16). e, Constrained ordination (ANOVA-like permutation test, full model, F2,255 = 6.156, R2 = 0.041, P = 0.001) indicated significant effects of legacy (by term, F1,255 = 4.254, P = 0.001) and test-phase water treatment (by term, F1,255 = 8.058, P = 0.001) on maize root bacterial composition. Ellipses indicate 95% confidence intervals. f, Acute drought increased maize root microbiota within-group variation (ANOVA, F1,262 = 20.65, P = 0.000008). All ANOVAs used type III sums of squares.
