Fig. 5: Mhp and ThsB1 are sufficient to stimulate ThsB2 cyclase activity in vitro.
From: Bacterial TIR-based immune systems sense phage capsids to initiate defense
a, HPLC analysis of the products resulting from the incubation of purified His6-ThsB1, ThsB2-His6 or both with NAD+ and Mhp. RT of reactants and products is marked with dashed lines. b, The same as in a but after incubation of both His6-ThsB1 and ThsB2-His6, alone (−) or in the presence of purified wild type or V273A mutant Mhp. c, HPLC analysis of the products resulting from the incubation of purified His6-ThsA with (+) or without (−) commercially available 1″-3′-gcADPR. RT of reactants and products is marked with dashed lines. d, HPLC analysis of the products resulting from the incubation of purified His6-ThsA with the products of the reactions shown in b, obtained after mixing ThsB2-His6, ThsB2F6A-His6, NAD+ and wild type or V273A mutant Mhp. RT of reactants and products is marked with dashed lines. ADPR, adenosine diphosphate ribose; NAM, nicotinamide.
