Extended Data Fig. 2: Mhp is required for Sau–Thoeris activation.
From: Bacterial TIR-based immune systems sense phage capsids to initiate defense
(a) Tenfold serial dilutions of Φ80a-vir phage carrying wild type, V273A, W84K mhp alleles on lawns of S. aureus RN4220 harboring plasmids carrying either an incomplete (thsB1/B2) or full (thsA/B1/B2) Thoeris operon. (b) Schematic of ΦNM1 genome, with expansion of the operon for packaging the genome and assembly of phage particles. PE and PL are promoters responsible for the expression of early and late viral genes, respectively. The green asterisk indicates the nonsense mutation prevents ΦNM1 lysogeny, converting it into ΦNM1g6, a purely lytic phage. Regions of the genome involved in different stages of the viral lytic cycle are indicated. (c) Genes of the ΦNM1 genome required for capsid biogenesis. (d) Tenfold serial dilutions of lysates obtained after MMC induction of different ΦNM1 lysogens carrying deletions on genes involved in packaging the genome and assembly of phage particles on lawns of S. aureus RN4220 harboring an empty vector control, or rescue plasmids expressing the genes deleted in the prophages.
