Extended Data Fig. 4: Hydrogen-deuterium exchange/mass spectrometry (HDX) analysis of prefusion-stabilized and postfusion gB ectodomains.
From: Prefusion structure, evasion and neutralization of HSV-1 glycoprotein B
a, Peptide coverage and differential hydrogen-deuterium exchange for prefusion and postfusion gB ectodomains determined by mass spectroscopy analysis. The regions are coloured according to the legend for relative protection in either conformation: red regions are more protected from hydrogen-deuterium exchange in prefusion gB, and blue regions are more protected in postfusion gB. b, Mapping of hydrogen-deuterium exchange differences onto prefusion (left) and postfusion (right) structures to highlight conformational changes. c, Representative uptake plots for the numbered regions in a and b, with structural details on the right. These regions exchange differently in the two gB states. Peptide 4 has bimodal exchange behaviour, suggesting two conformations in solution. n = 2 technical replicates (shapes). See Supplementary Figs. 2-4 for additional information on HDX/MS experiments and analysis.
