Extended Data Fig. 3: Detection of HSV-1 gB binding and HSV-1 neutralizing responses.
From: Prefusion structure, evasion and neutralization of HSV-1 glycoprotein B
a-c, ELISA binding (top) and authentic HSV-1 KOS neutralization (bottom) by week 5 sera from immunized mice, divided by immunization group (n = 10 per group). Immunogen and mouse strain are listed at the top of each column. Top, mean ELISA EC50 values for each conformation of gB are listed under each set of data, with each data point indicating the mean value from two technical replicates. Bottom, mice with low levels of detectable neutralization (reduction of cytopathic effect; CPE) are boxed: one preF-immunized BALB/c, two preF-immunized BL/6, and three postF-immunized BL/6. Each point represents the mean value of technical triplicates with error bars depicting the standard deviation. d, Direct comparison of ELISA EC50 titres elicited in BL/6 mice to highlight that postF gB is more immunogenic than preF gB. Error bars depict standard deviation, together with a measure of the mean for each group. Statistical differences were determined by two-tailed Student’s t-test. e, Neutralization of authentic HSV-1 KOS by monoclonal antibody D48 and the pooled human IgG reagent, Flebogamma. VRC01 is an HIV-neutralizing monoclonal antibody used as a negative control. Each point represents the mean value of technical triplicates, with error bars depicting the standard deviation.
