Extended Data Fig. 4: Expression profiles of Or43b and Or74a in D. melanogaster.
From: Engineered Metarhizium fungi produce longifolene to attract and kill mosquitoes
(a) RT-PCR analysis of Or43b and Or74a expression in larvae and adult antennae. Larva: total RNA from the most anterior part of the larvae, containing dorsal organ; Adult: total RNA from antennae. M: DNA ladder. Actin5C: reference gene. (b) Determination of the expression of Or43b and Or74a in larvae via observation of mRFP in a single symmetric pair of larval ORNs (Arrowed), one in each dorsal organ in larvae with mRFP expression driven by Or43b-GAL4 (genotype: Or43b > mCD8-RFP) (Upper panel) and Or74a-GAL4 (genotype: Or74a > mCD8-RFP) (Lower panel). The most anterior part of Drosophila larva outlined with white dash line. Scale bars: 20 μm. Images are representative of three independent experiments.
