Fig. 2: The AIBP vaccine promotes DC maturation and production of TH1- and TH17-polarizing cytokines.
a,b, Bone marrow-derived DCs were stimulated for 24 h with AIBP or wP vaccine (bacterium-to-cell ratio of 10:1) or medium (Med.). a, Surface expression of MHCII, CD40 and CD80 was evaluated using flow cytometric analysis gated on live single cells. Results are expressed as mean fluorescence intensity (MFI) and show individual values for four biological replicates. b, Concentrations of IL-1β, IL-12p70 and IL-23 in supernatants were quantified by ELISA (n = 3). c, Groups of eight female 6–8-week-old C57BL/6 mice were immunized by exposure to an aerosol of the AIBP vaccine or intramuscularly with a wP vaccine or PBS. After 4 h and 24 h, concentrations of IL-1β and IL-23 in lung homogenates were quantified by ELISA. d, CD4 T cells enriched from the spleen of B. pertussis convalescent mice were cultured with AIBP or wP vaccines at concentrations equivalent to 10 bacteria to one DC, and after 3 days, IL-17 was quantified in supernatants by ELISA. Data are presented as the mean ± s.e.m. of four biological replicates shown as individual symbols. Data were analysed by one-way ANOVA followed by Tukey’s test for multiple comparisons. P values are shown above relevant datasets.
