Fig. 4: Previous immunization of mice with aP vaccines does not affect the induction of TH1 and TH17 cells or the efficacy of the AIBP vaccine.
a, Schematic of the experiment; mice were immunized intramuscularly with the aP vaccine (twice at 0 week and 4 weeks; 1/50 of the human dose) followed by aerosol administration of the AIBP vaccine (twice, 8 weeks and 12 weeks) or given two doses of the aP or AIBP only or PBS. Female 6–8-week-old C57BL/6 mice were aerosol challenged with live B. pertussis at week 14. b,c, On the day of but before the challenge with live B. pertussis, CD4 TRM cells (b) and B. pertussis-specific IL-17- and IFNγ-producing CD4 TRM cells (c) were analysed by ICS and flow cytometry as described in the legend of Fig. 3. Data in b and c are presented as the mean ± s.e.m. of biological replicates shown as individual symbols (n = 5). d, On the day before the B. pertussis challenge, concentrations of B. pertussis-specific IgA, IgG1 and IgG2c in nasal tissue homogenates and serum were quantified by ELISA. Data are presented as the mean ± s.e.m. of biological replicates (n = 5). e, CFU counts on lung and nasal tissue 2 h, 7 days, 14 days and 21 days post-challenge. Data are presented as the mean ± s.e.m. of biological replicates (n = 5). Data were analysed by two-way ANOVA followed by Tukey’s test for multiple comparisons. P values are shown above relevant datasets. Panel a created with BioRender.com.
