Extended Data Fig. 3: Representative cryo-EM data processing scheme.
From: Structural and mechanistic insights into herpesvirus helicase–primase and its therapeutic inhibitors
Summary of the major steps of cryo-EM data processing for the HSV-1 HP complex with the AMNV dataset. The flowchart outlines each step in the process. Scale bars represent 20 Å in the micrographs and 130 Å in the 2D classification average. The gold-standard Fourier shell correlation (FSC) curve54 shows an overall resolution of 2.6 Å at an FSC of 0.143, for the consensus map generated by CryoSPARC NU refinement. Transparent yellow and cyan surfaces represent the two masks used to separate the rigid and flexible bodies for Multibody refinement. FSC curves for each body’s resolution, calculated in Relion Multibody refinement, are shown with designated lines. The final composite map used in Fig. 1a is also displayed.
