Extended Data Fig. 6: CRISPR–Cas subtype III-I.
From: An updated evolutionary classification of CRISPR–Cas systems including rare variants
a, Comparison of representative typical III-D2, III-E and III-I loci organization. Cas7 and Cas11 domains within large multidomain proteins are shown by boxes within the respective arrows. b, Dendrograms for individual Cas7 domains. The larger tree is a hybrid UPGMA/FastTree tree built for all best hits obtained by PSI-BLAST search with the three individual Cas7 domain of Cas7-11i used as queries. The smaller UPGMA dendrogram was built using a matrix of Pairwise rmsd scores as obtained by DALI comparison for individual Cas7 domain from the Cas7-11i AF3 model, Cas7-11e structure (7zol), AF3 model for GwCas7*3 28 and the Cas7 domain from the III-D effector complex structure (8bmw). Underneath the trees, a scheme of similarity between Cas7 domain of Cas7-11i and Cas7-11e are shown. Solid line indicates Cas7 domains with structural similarity and dashed line shows domains with significant sequences similarity. c, AF3 model for Cas7-11i (WP_207677910.1) and Cas10i (WP_207677911.1) complexed with crRNA from subtype III-D. Cas7 and putative Cas11 domain are coloured, Cas10i is shown in blue. d, Structural alignment of AF2 model of Cas10i and Cas10d structure (8s9t-C). Below the DALI structure-guided alignment, the alignment of the catalytic motif (GGDD) of Cas10 and the corresponding region of Cas10i, demonstrating the disruption of the catalytic site in the latter.
