Fig. 4: Influenza virus peptide pool-stimulated IFNγ secretion.
Secreted IFNγ from the PBMC supernatants were measured using Luminex assay and the data are presented as stimulation index (SI). a, SI values for H5-stimulated PBMC supernatants. b, SI values for H1-stimulated PBMC supernatants. c, SI values for N8-stimulated PBMC supernatants. d, SI values for NP-stimulated PBMC supernatants. As positive controls, PBMCs were stimulated with SARS-CoV-2 JN.1 variant spike peptide pool and tetanus toxoid (TET) e, SI values for JN.1 variant spike peptide pool-stimulated PBMC supernatants. f, SI values for tetanus toxoid-stimulated PBMC supernatants. IFNy data were categorized into two groups: previously unvaccinated (n = 13) and previously vaccinated (n = 5), at two time points: pre-vaccination and 3 weeks after the second dose. The graphs display geometric mean indices (lines) and 95% CIs (whiskers). The dashed line indicates the positivity threshold, which was considered to be SI 2. Only statistically significant differences between time points within groups are indicated. Comparisons within a group between two time points were conducted using two-sided Wilcoxon matched-pairs signed-rank test.
