Extended Data Fig. 4: Split-luciferase assay.
From: Pneumococcal S protein coordinates cell wall modification and repair to resist host antimicrobials
(A) All proteins tested by split-luciferase assay. High relative luminescence unit (RLU, normalized log) indicate close proximity (0 represent no proximity, 3 represent close proximity), see material and methods for details of normalization. HlpA-LgBit HlpA-SmBit (HlpA-HlpA) is used as a positive control. Protein representations are not drawn to scale, and when relevant, C and N indicate the sub-cellular localization of the C- and N-terminal SmBit fusion respectively. (B) Fusion of the LgBit to the C-ter of MpgA and RodZ confirmed their close proximity to S protein.
