Extended Data Fig. 5: Pneumococcal PBP1a and PgdA are co-localizing and the removal of the S protein does not impact their localization.
From: Pneumococcal S protein coordinates cell wall modification and repair to resist host antimicrobials
Double-labeled cells were grown in C + Y medium at 37°C and mid-exponentially growing cells were collected for fluorescence microscopy. Fluorescent proteins were expressed from their native locus as only copy in the cell. (A) Deconvolved epifluorescence microscopy. Scale bar = 2 µm. (B) Fluorescent signal of one replicate from >1,000 cells per strain are ordered by cell length and represented by demographs plot performed using MicrobeJ40 (see Methods). (C-D) same as A and B but in an ess deleted background.
