Extended Data Fig. 9: Deacetylated muropeptides are reduced in a S protein mutant.
From: Pneumococcal S protein coordinates cell wall modification and repair to resist host antimicrobials
(A) Muropeptide profiles of exponentially growing cells in C + Y medium at 37°C were obtained by reversed-phase HPLC55. The area under each peak (numbers) were calculated for each strain (Supplementary Table 5). Peak number 2, 17 and 18 correspond to Tri[deAc], TetraTri[deAc]‡, and TetraTri[deAc]‡, respectively55,94, as reported before by (Bui et al. 2011). Experiments were performed in duplicates and a single profile is shown for each strain. (B) Relative amount of deacetylated peaks of muropeptide profile done in duplicates. Also see (Supplementary Table 4, 5). (C) Pneumococcal susceptibility to host-induced damage in the absence of the S protein can be complemented and is also seen in pneumococcal cells lacking the capsule. Heatmap of the area under the curve (AUC) of the growth curves in liquid media. Lysozyme and LL-37 act synergistically on the ΔessSp mutant. Empirical AUC was plotted between 0-7 hours.
