Extended Data Fig. 4: Infection phenotypes of other S. zooepidemicus mutants.
a, Survival curves of animals challenged with 5×106 CFU of WT, ΔproB and ΔproC. Animals were monitored until moribund or 14 dpi. b, C57BL/6 J mice were intravenously injected with 5 × 106 CFU of either ΔproB or ΔproC, and bacterial counts in the blood were measured. ΔproB and ΔproC persists at a low level in the blood and were eliminated at 14 dpi. c, Survival curves of animals challenged with 5×106 CFU of Δ6210. Mice were monitored until moribund, or 120 hpi. d, Bacterial burden in organs and blood was assessed in moribund mice after i.v. infection with 5×106 CFU Δ6210 (n = 5) and WT (n = 6). e, Survival curves of animals challenged with 5×106 CFU WT, ΔmanY or CΔmanY via i.v. injection through tail vein. CFU data (b,d) are shown as geometric mean ± geometric s.d. Survival curves (a, c, e) were analyzed by log-rank test versus WT. CFU burden (d) was analyzed by two-way ANOVA followed by Šídák’s multiple comparisons test on log-transformed CFU.
