Extended Data Fig. 3: Construction and screening of transposon insertion library.
a, Circular visualization of SEZTn library. The purple and yellow rings represent unique transposon insertion counts at each TA dinucleotide site. The blue and red rings indicate coding regions on different DNA strands. Total TA site insertion counts are provided in Supplementary Table S12. b, Volcano plot of three rounds of SEZTn screening in the brain. Red points denote PTS components, and orange points denote select genes involved in proline synthesis. t-test was used to compare normalized reads between the average number of mapped reads for the input and output library to derive P value. All gene Fold change in three rounds of screening are provided in Supplementary Table S1. c, Comparison of the distributions of transposon insertions in another mannose PTS operon (RS06200 ~ RS06215) and the upstream and downstream gene in the input library, as well as in three rounds of the output samples. The RS06210 gene is marked in red. The yellow background denotes the coding sequence (CDS) and intergenic regions. TA sites indicate potential transposon insertion site. d, Comparison of the distributions of transposon insertions in PTSman operon and the upstream and downstream gene in the input library, as well as in three rounds of the output samples, which reveals underrepresentation of PTSman and its promoter (PmanX). The manY gene is marked in red. The yellow background denotes the CDS and intergenic regions of PTSman, and the blue background highlights the promoter regions of PTSman. TA sites indicate potential transposon insertion sites.
