Fig. 2: The B. divergens transcriptome reveals expression of putative egress and invasion genes.

a, Expression profile of all differentially expressed genes. b, Number of genes identified by both transcriptomic approaches. Differentially expressed genes are defined as having a >1.5-fold change over time for the bulk transcriptome and a ≥2 fold change, adjusted P value 0.001, for the single-cell transcriptome (see Methods for details). Green and purple bars represent data from the single-cell and bulk synchronous transcriptomes, respectively. The numbers within the bars show the total number of genes identified and the percentage of genes this represents from all genes. c, Correlation of timing of peak expression between the bulk and single-cell transcriptomes. Error shown is the 95% confidence interval (CI) of the fitted linear regression. Statistical analysis was conducted using a two-sided t-test for the regression slope. d, The expression profiles from bulk synchronous and single-cell data of orthologues of known egress and invasion genes. Some genes are not present in the single-cell analysis owing to lower sensitivity to low-abundance transcripts. e, The expression profiles from bulk synchronous data of putative egress and invasion genes. The single cell data were originally generated in ref. ³⁸.