Table 1 IC50 values of aspartyl protease inhibitors against B. divergens
From: Babesia divergens host cell egress is mediated by essential and druggable kinases and proteases
|  | Wild-type IC50 (±s.d.) (µM) | ASP2 (high Shld1) IC50 (±s.d.) (µM) | ASP2 (low Shld1 = knockdown) IC50 (±s.d.) (µM) | ASP3 (high Shld1) IC50 (±s.d.) (µM) | ASP3 (low Shld1 = knockdown) IC50 (±s.d.) (µM) |
|---|---|---|---|---|---|
CWHM-0000032 | 0.891 (±0.307) | 0.320 (±0.212) | 0.478 (±0.288) | 0.362 (±0.129) | 0.246 (±0.095) |
CWHM-0000033 | 4.03 (±1.90) | 1.57 (±0.17) | 1.63 (±0.94) | 1.39 (±0.26) | 0.539 (±0.358) |
CWHM-0000099 | >25 | 11.8 (±0.8) | 3.99 (±3.15) | 16.3 (±3.7) | 2.85 (±1.52) |
CWHM-0000117 | 6.81 (±3.08) | 2.66 (±0.55) | 2.56 (±1.32) | 3.17 (±0.61) | 1.13 (±0.50) |
CWHM-0000166 | 8.80 (±3.18) | 2.89 (±1.27) | 1.15 (±0.70) | 2.76 (±0.96) | 0.337 (±0.157) |
CWHM-0000047 | 19.5 (±5.3) | 18.5 (±12.9) | 6.32 (±5.84) | 16.9 (±9.0) | 8.47 (±5.35) |
CWHM-0000579 | 12.6 (±6.6) | 7.49 (±4.73) | 11.1 (±6.5) | 9.12 (±6.12) | 7.32 (±6.48) |
CWHM-0000162 | 13.3 (±9.4) | 7.15 (±6.45) | 6.47 (±5.35) | 8.00 (±5.06) | 6.41 (±6.74) |
SLU-0010620 | 19.8 (±5.3) | 10.1 (±3.4) | 4.67 (±3.06) | 14.1 (±2.6) | 10.0 (±5.4) |
SLU-0010622 | 10.9 (±3.3) | 6.38 (±3.27) | 1.89 (±2.78) | 8.04 (±3.36) | 5.49 (±4.29) |
CWHM-0000068 | 16.6 (±7.6) | 15.7 (±12.6) | 8.02 (±0.89) | 13.3 (±2.9) | 9.88 (±4.84) |
CWHM-0000116 | >25 | >25 | 18.8 (±8.2) | 20.3 (±11.3) | 21.4 (±10.2) |
CWHM-0000123 | 9.46 (±3.66) | 8.39 (±4.39) | 10.8 (±2.8) | 11.2 (±8.7) | 9.22 (±1.76) |
CWHM-0000293 | >25 | >25 | >25 | >25 | >25 |
CWHM-0000299 | >25 | >25 | >25 | >25 | >25 |
CWHM-0000460 | >25 | 12.3 (±10.2) | 12.2 (±1.5) | 12.4 (±5.6) | 12.6 (±4.0) |
CWHM-0000580 | >25 | >25 | >25 | >25 | >25 |
CWHM-0000583 | >25 | 14.2 (±9.5) | 14.5 (±8.9) | 13.8 (±5.1) | 14.1 (±6.9) |
CWHM-0000658 | 16.2 (±9.8) | 12.6 (±11.9) | 6.47 (±3.82) | 9.35 (±3.77) | 7.82 (±5.45) |
SLU-0010621 | 25.7 (±12.3) | 15.8 (±9.6) | 8.64 (±4.98) | 15.8 (±3.6) | 18.6 (±13.4) |
SLU-0010623 | 24.2 (±10.7) | 6.37 (±3.46) | 2.57 (±2.67) | 13.4 (±7.2) | 12.1 (±8.2) |
SLU-0010624 | 18.5 (±5.7) | 6.38 (±3.72) | 5.76 (±6.77) | 9.62 (±4.22) | 9.30 (±6.24) |
UCB7362 | 0.908 (±0.232) | 1.21 (±0.76) | 0.764 (±0.306) | 0.113 (±0.021) | 0.020 (±0.012) |
WM382 | 0.0341 (±0.0064) | 0.00775 (±0.0019) | 0.00267 (±0.0014) | 0.00695 (±0.0010) | 0.00088 (±0.0005) |
ML10 | 0.864 (±0.319) | 0.794 (±0.072) | 1.56 (±1.24) | 0.682 (±0.256) | 0.628 (±0.219) |
Atovaquone | 0.021 (±0.007) | 0.020 (±0.005) | 0.020 (±0.008) | 0.021 (±0.006) | 0.025 (±0.011) |
