Extended Data Fig. 2: LRP8 promotes YF17D infection in K562 cells.
a, b. Ectopic LRP8 (a) and luciferase (b) expression on K562 cell surface were determined by FACS with HA antibody staining. c. In K562 cells, HA-tagged luciferase and LRP8 were stably overexpressed and the cells are infected by YFV 17D pseudoviruses and the infection rates were determined by FACS at 24 hpi. d. In K562 cells, HA-tagged luciferase and LRP8 were stably overexpressed and the cells are infected by live YFV 17D and viral RNA level in cells were determined by RT-qPCR at 48 hpi. e. Infectious viral particles from d were determined by plaque assay. Statistical analysis was performed using two-way analysis of variance (ANOVA) with Dunnett’s multiple comparisons test(c-e). The statistics shown are Mean ± SEM. NS: not significant. The replicate in the figure indicates biological replicates, the experiments were repeated at least twice.
