Fig. 1: Synthesis and biological evaluation of ABPP probes and 5-nitroimidazole derivatives. | Nature Microbiology

Fig. 1: Synthesis and biological evaluation of ABPP probes and 5-nitroimidazole derivatives.

From: Metronidazole and ether derivatives target Helicobacter pylori via simultaneous stress induction and inhibition

Fig. 1: Synthesis and biological evaluation of ABPP probes and 5-nitroimidazole derivatives.

a, The general mode of action of Metro in bacteria: (1) cell entrance via passive diffusion, (2) bioactivation of nitro group by respective electron-transport system and redox enzymes, (3) reaction of reactive radical species with cellular components such as DNA or proteins and (4) reoxidation to parent compound via ‘futile cycling’ in the presence of oxygen in aerobic bacteria. b, An overview of synthesized ABPP probes Metro-P1, Metro-P2, Metro-P3 and Metro-P4 and respective MIC values in H. pylori 26695. c, An overview of synthesized 5-nitroimidazole ether derivatives. d, The correlation plot of log MIC values to reduction potential determined via simple linear regression. e, A table showing MIC values of derivatives in H. pylori 26695 (n = 3) and their reduction potential Ered against NHE. Dimetridazole-2-hydroxy and metronidazol are included for reference. Schematic in panel a partially created with BioRender.com.

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