Extended Data Fig. 9: Sequencing analysis of T4 and plasmid DNA fragments incubated with nuclease-NTPase systems reveals degenerate cleavage motifs. | Nature Microbiology

Extended Data Fig. 9: Sequencing analysis of T4 and plasmid DNA fragments incubated with nuclease-NTPase systems reveals degenerate cleavage motifs.

From: Nuclease–NTPase antiphage defence systems use conserved molecular features to control bacterial immunity

Extended Data Fig. 9: Sequencing analysis of T4 and plasmid DNA fragments incubated with nuclease-NTPase systems reveals degenerate cleavage motifs.The alternative text for this image may have been generated using AI.

a, c, d, f, Fragment size and position graphs for PaAbpAB (a), BmAzaca (c), BtHachiman (d), and EcPD-T4-8 (f) cleavage reactions with phage T4 DNA reveals that each system cuts throughout the length of T4 DNA. b, e, g, WebLogos, consensus sequences, fragment size and position graphs for PaAbpAB (b), BtHachiman (e), EcPD-T4-8 (g) cleavage reactions with plasmid DNA. All three systems exhibit degenerate cleavage site motifs and cut throughout the length of plasmid DNA. All reactions were optimized such that fragment sizes would be between 150–350 bp for sequencing.

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