Extended Data Fig. 10: 10-oxoSA effects on bacteria growth and 10-oxoSA production.
From: Microbial 10-oxostearic acid protects mice against colitis via the nuclear receptor PPARα
a Relative fecal bacterial abundance (n = 11 for Vehicle, n = 12 for 10-oxoSA). Data are expressed as means ± SD. P values were calculated using a two-sided Wilcoxon rank-sum test. Green indicates the species inhibited by 10-oxoSA. Red indicates the species promoted by 10-oxoSA. b,c Growth of representative A. muciniphila, L. reuteri NM11, L. reuteri NM12 and L. johnsonii NM60 strains supplemented with 150 μg/ml of 10-oxoSA. F. prausnitzii, R. gnavus, and C. scindens are controls. n = 4 for A. muciniphila, F. prausnitzii, R. gnavus, and C. scindens, n = 6 for L. reuteri NM11, and L. reuteri NM12, n = 5 for L. johnsonii NM60. Data were analyzed by two-way ANOVA with multiple comparisons and are expressed as mean ± SD. d,e Evaluation of 10-oxoSA, and 10-HSA producing ability across a 27-strain lactobacilli collection. Lactobacilli strains were incubated for 48 hours with (d) OA (500 µM) or (e) LA (500 µM) in MRS broth, and the negative control without OA (n = 3 technical replicates). Values were normalized to bacterial cell density by OD600 and corrected by subtraction of the negative control value. Data are expressed as means ± SD. 10-HODA 10-hydroxy-12-octadecenoic acid. Source data are provided in the Source Data file.
