Fig. 1: Selection for 3BNC117 or 10-1074 resistance across a diverse panel of HIV-1 strains.
From: Diverse paths to broadly neutralizing antibody escape among HIV-1 strains
a, Phylogeny of env coding sequences from primary HIV-1 strains from subtypes A, B, C and D. The 3BNC117 and 10-1074 IC80 value (in µg ml−1) is indicated to the right of each strain. Viruses included in this study are indicated with asterisks. b, An overview of the experimental strategy used to identify putative 3BNC117 or 10-1074 resistance mutations. c, An example of data obtained with selection of the SUMA virus strain at 10 µg ml−1 3BNC117. The blue line represents parental virus growth kinetics in the absence of 3BNC117 and the orange lines are resistant isolates emerging over time. d, An example of sequencing and phenotypic analysis of the SUMA strain as in c. AA, amino acid. e, The estimated frequency of bnAb-resistant variants for 3BNC117 and 10-1074 in the starting virus population before selection, derived from the number of starting viruses screened and the number of wells where virus replication was measured. *P < 0.05; n.s., not significant.
