Extended Data Fig. 6: Uncommon 3BNC117 escape pathways.
From: Diverse paths to broadly neutralizing antibody escape among HIV-1 strains
a, Titer of CH607 isolates grown in the absence of 3BNC117 (orange) or selected with 1μg/mL 3BNC117 (pink) in comparison to all other virus isolates obtained following selection with 3BNC117 (blue). b-c, Neutralization curves of viruses pseudotyped with CH607 Env mutants isolated after selection with 3BNC117 at 1μg/mL. Algorithmically identified putative resistance mutations are show in b and combinations found in individual isolates are shown in c. d, 293Ts were transfected with an infectious molecular clone of CH607 bearing the indicated mutant and co-cultured with MT4-R5-GFP cells at a 3BNC117 concentration of 10 µg/mL. Relative infection was calculated for each mutant and violin plots show the distribution of relative infection values generated from all replicate combinations (34 = 81 per mutant, see Methods). Horizontal bars indicate the bootstrap mean and error bars represent the 95% resampling confidence interval.e, Electrostatic potential calculated for parental (left) or mutant (right) CH607 Env surface (PDB 5v8m). f, Number of 3BNC117 resistant 9004SDM isolates with the indicated selected amino acid substitution. 3BNC117 IC80 values were determined for selected mutants as indicated.
