Fig. 4: Unusual mechanisms of 3BNC117 resistance in three HIV-1 strains involve enhanced cell-to-cell transmission or accumulation of multiple amino acid substitutions.
From: Diverse paths to broadly neutralizing antibody escape among HIV-1 strains
a, The 293T cells were transfected with an IMC of CH607 bearing the indicated mutation and co-cultured with MT4-R5-GFP cells in the presence of 3BNC117 at 1 µg ml−1. Relative infection was calculated for each mutant and violin plots show the distribution of relative infection values generated from all replicate combinations (34 = 81 per mutant; Methods). Horizontal bars indicate the bootstrap mean and error bars represent the 95% resampling confidence interval. b, The experimental set up as in a, except 293T and MT4-R5-GFP cells were placed in different compartments of a 0.2-µm transwell. c, The 3BNC117 IC80 values against viruses pseudotyped with wild-type 9004SDM Env or those carrying the indicated V5-loop deletions selected in our experiments. d, The 3BNC117 IC80 values against viruses pseudotyped with TRO.11 Env bearing the indicated mutations. The red gradient represents the range of values obtained for the wild-type TRO.11 Env over multiple experiment replicates. Each dot represents an independent experimental replicate for each indicated mutational combination.
