Extended Data Fig. 5: The tunicamycin condition has lower signal to noise ratio, resulting in downstream bottlenecks when comparing conditions. | Nature Microbiology

Extended Data Fig. 5: The tunicamycin condition has lower signal to noise ratio, resulting in downstream bottlenecks when comparing conditions.

From: Pooled CRISPRi screening reveals fungal-specific drug target candidates

Extended Data Fig. 5: The tunicamycin condition has lower signal to noise ratio, resulting in downstream bottlenecks when comparing conditions.The alternative text for this image may have been generated using AI.

a) The number of hit sgRNAs for a specific condition is negatively correlated with signal to noise ratio. For each condition, we computed the difference between the 5% FDR Log2FC threshold and the median Log2FC of the five most depleted sgRNAs. Correlation was measured using two-sided Spearman’s rank correlation (n = 11 conditions). Data points are colored by condition type as shown on the left. b) Number of conditions with significant fitness decreases for hit genes in the experiment, with or without including the tunicamycin condition. c) Number of depleted sgRNAs per gene in each condition, with tunicamycin highlighted in green. Only genes which had hits in at least two conditions are shown.

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