Extended Data Fig. 1: DSPE-PCB/insulin formulations with increasing zinc content showed increased retention of insulin release. | Nature Nanotechnology

Extended Data Fig. 1: DSPE-PCB/insulin formulations with increasing zinc content showed increased retention of insulin release.

From: Zwitterionic micelles efficiently deliver oral insulin without opening tight junctions

Extended Data Fig. 1

DSPE-PCB/insulin formulations were dialyzed (10 kDa MWCO) against pH 1.2 and 6.8 buffer with 5 mM bile salt at 37 °C. Formulation 1, 2, and 3 had an insulin/ZnCl2 feeding ratio of 50/1, 20/1, and 2.5/1 by weight during the encapsulation process, respectively. Their drug loading is 6.24%, 6.23% and 6.10%, while the corresponding particle sizes are 28.52, 26.36 and 25.96nm respectively. The cumulative released insulin was measured using the BCA assay (N=3 independent experiments, means connected).

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