Extended Data Fig. 7: E64-DNA does not activate T cells through allostimulation or direct stimulation.
From: A lysosome-targeted DNA nanodevice selectively targets macrophages to attenuate tumours
a-b, Control for allostimulation. CD8+ T cell activation (a) and proliferation (b) after 72 h of co-culture with E64-DNA-treated (100 nM) TAMs that had not been exposed to antigen. CD3/CD28 antibodies were included as a positive control for T cell activation. n = 3/group. c-d, Control for direct effects of E64-DNA on T cells. CD8+ T cell activation (c) and proliferation (d) after 72 h of culturing in complete growth media (Media) in the presence/absence of E64-DNA (100 nM). CD3/CD28 antibodies were included as a positive control for T cell activation. n = 3/group. Statistical significance was calculated via two-tailed Student’s t-test (P < 0.05 values are provided); error bars indicate the mean of independent experiments ± s.e.m. All measurements (n) are biological replicates.
