Extended Data Fig. 1: TFEB is responsible for elevated lysosomal enzymes in M2-like macrophages.
From: A lysosome-targeted DNA nanodevice selectively targets macrophages to attenuate tumours
a, Validation of lysosomal proteins elevated in M2 BMDMs by immunoblotting, related to Fig. 1d. Representative of 2 independent experiments. b, mRNA levels of lysosomal genes in M1 and M2 BMDMs. n = 3/group. c, Tfeb mRNA levels in M1 and M2 BMDMs. n = 3/group. d, Immunoblot of TFEB protein levels in M1 and M2 BMDMs. Representative of 3 independent experiments. e, Immunoblot of cytosolic and nuclear TFEB levels in M1 and M2 BMDMs. Representative of 2 independent experiments. f, Validation of mTfeb-/-. mRNA levels (top) n = 3/group and protein levels (bottom). Representative of 3 independent experiments. g, A comparison of lysosomal gene expression in M1 and M2 BMDMs from fl/fl mice versus M2 BMDMs from mTfeb-/- mice, n = 3/group; and a comparison of lysosomal gene expression in TAMs from fl/fl and mTfeb-/- E0771 tumors, n = 4/group. h, DQ-OVA degradation assays of fl/fl and mTfeb-/- M2 BMDMs. n = 3/group. Statistical significance was calculated via two-tailed Student’s t-test (P < 0.05 values are provided); error bars indicate the mean of independent experiments ± s.e.m. All measurements (n) are biological replicates.
