Extended Data Fig. 2: TAMs exhibit increased lysosomal enzyme levels and activity.
From: A lysosome-targeted DNA nanodevice selectively targets macrophages to attenuate tumours
a, Isolation of mammary ATMs from tumor-free mice and TAMs from E0771 mammary tumor-bearing mice. Purity of ATMs and TAMs was validated by flow cytometry. b, Immunoblots of lysosomal protein levels in ATMs and TAMs. Experiment was performed once with n = 3/group. c, DQ-OVA degradation assays of ATMs and TAMs. n = 3/group. d, mRNA expression of lysosomal genes in TAMs isolated from E0771 tumors and thioglycolate-elicited peritoneal macrophages from tumor-free mice. n = 3/group. Statistical significance was calculated via two-tailed Student’s t-test (P < 0.05 values are provided); error bars indicate the mean of independent experiments ± s.e.m. All measurements (n) are biological replicates.
