Fig. 2: Screen of diverse NP biodistributions in naive and i.v.-LPS-affected lungs. | Nature Nanotechnology

Fig. 2: Screen of diverse NP biodistributions in naive and i.v.-LPS-affected lungs.

From: Supramolecular arrangement of protein in nanoparticle structures predicts nanoparticle tropism for neutrophils in acute lung inflammation

Fig. 2: Screen of diverse NP biodistributions in naive and i.v.-LPS-affected lungs.The alternative text for this image may have been generated using AI.

a–c, NAPs accumulate in acutely inflamed lungs. a, Biodistributions of variant NGs indicating uptake of 75 nm NGs (n = 4 i.v.-LPS animals, n = 4 naive animals, red box: P < 1 × 10−10) and 200 nm NGs (n = 5 i.v.-LPS, n = 5 naive, red box: p < 1 × 10−10) in LPS-injured lungs, but not naive lungs. Data for 130 nm NGs are identical to that presented in Fig. 1b. b, Biodistributions of variant crosslinked albumin NPs indicating uptake of albumin nanorods (n = 3 i.v.-LPS animals, n = 3 naive animals; red box, P < 1 × 10−10) and bovine albumin NPs (n = 3 i.v.-LPS animals, n = 3 naive animals; red box, P < 1 × 10−10) in LPS-injured, but not naive lungs. Data for human albumin nanoparticles are identical to that presented in Fig. 1c. c, Biodistributions of charge-agglutinated protein NPs, indicating uptake of particles comprised of E-GFP and guanidine-tagged PONI or particles comprised of E-GFP and guanidine-tagged gold nanoparticles in LPS-injured (PONI: n = 5 animals; Au: n = 3 animals), but not naive (PONI: n = 4 animals; Au: n = 3 animals) lungs. PONI/E-GFP data reflect tracing of both 131I-labelled PONI and 125I-labelled E-GFP. For PONI tracer data: red box, P < 1 × 10−10. For E-GFP tracer data: red box, P = 0.0003. For Au/E−GFP data: red box, P = 1.6 × 10−9. d, NPs based on symmetric supramolecular arrangement of protein do not have tropism for inflamed lungs (schematics created with BioRender.com). Biodistributions of adenovirus (n = 5 i.v.-LPS animals, n = 5 naive animals; blue box, P = 0.88), adeno-associated virus (n = 3 i.v.-LPS animals, n = 3 naive animals; blue box, P = 0.56) and ferritin nanocages (n = 5 i.v.-LPS animals, n = 5 naive animals; blue box, P = 0.35) indicating no selectivity for LPS-injured versus naive lungs. e, Biodistributions of bare liposomes (schematic created with BioRender.com, n = 4 i.v.-LPS animals, n = 4 naive animals) indicating no selectivity for LPS-injured versus naive lungs (blue box, P = 0.31). Biodistributions of IgG-coated polystyrene NPs indicating low levels of uptake in both naive (n = 4 animals) and LPS-injured (n = 4 animals) lungs (blue box, P = 0.0004). Statistical significance in all panels is derived from two-way ANOVA with Sidak’s multiple comparisons test. All error bars indicate mean ± s.e.m.

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