Fig. 3: Engineering of liposome surface chemistry to confer NAP-like behaviour in LPS-inflamed lungs. | Nature Nanotechnology

Fig. 3: Engineering of liposome surface chemistry to confer NAP-like behaviour in LPS-inflamed lungs.

From: Supramolecular arrangement of protein in nanoparticle structures predicts nanoparticle tropism for neutrophils in acute lung inflammation

Fig. 3: Engineering of liposome surface chemistry to confer NAP-like behaviour in LPS-inflamed lungs.The alternative text for this image may have been generated using AI.

a, Schematic of antibody-coated liposomes prepared via copper-free click reaction of azide-functionalized liposomes with DBCO-functionalized IgG (liposome schematic created with BioRender.com). b, Biodistributions in i.v.-LPS-injured mice for bare liposomes (n = 3 animals), liposomes conjugated to IgG via SATA-maleimide chemistry (n = 3 animals) and liposomes conjugated to IgG via DBCO–azide chemistry (n = 3 animals) (red box, P < 1 × 10−10 for DBCO–IgG liposomes versus bare liposomes and DBCO–IgG liposomes versus SATA–IgG liposomes). c, Mouse lung flow cytometry data indicating Ly6G anti-neutrophil staining density versus levels of DBCO–IgG liposome uptake as indicated by the green fluorescent TopFluor PC lipid signal. d, Flow cytometry data verifying increased DBCO–IgG liposome uptake in and selectivity for neutrophils following LPS insult. Inset: verification of increased concentration of neutrophils in the lungs following LPS. e, Fraction of neutrophils positive for DBCO–IgG liposomes in naive (n = 3 animals) or i.v.-LPS-injured (n = 3 animals) lungs (*P = 0.0003) and fraction of DBCO–IgG liposome-positive cells that are neutrophils (*P = 1.7 × 10−6). f, Biodistributions in i.v.-LPS-injured mice for azide-functionalized liposomes conjugated to IgG loaded with 2.5 (n = 3 animals), 5 (n = 3 animals), 10 (n = 4 animals) and 20 DBCO molecules per IgG (n = 3 animals; red box, P < 1 × 10−10 for DBCO(20×)–IgG liposomes compared to each of the other DBCO density groups). Insets in b and f: ratio of NP uptake in the lungs to NP uptake in the liver. Statistical significance in b and f is derived from two-way ANOVA with Tukey’s multiple-comparisons test. Statistical significance in e is derived from paired two-tailed t tests. All error bars indicate mean ± s.e.m.

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