Fig. 5: Sample-to-result workflow, using sample cartridge for extraction-free sample preparation, followed by plasmonic thermocycling integrated with real-time multispectral fluorescence monitoring. | Nature Nanotechnology

Fig. 5: Sample-to-result workflow, using sample cartridge for extraction-free sample preparation, followed by plasmonic thermocycling integrated with real-time multispectral fluorescence monitoring.

From: Multiplexed reverse-transcriptase quantitative polymerase chain reaction using plasmonic nanoparticles for point-of-care COVID-19 diagnosis

Fig. 5

a, Image of a custom sample cartridge (entire cartridge is 5.60 in. × 0.81 in.). Disassembled components of the cartridge are shown, including the body, reaction module, plunger and shroud. b, Pre-analytical workflow for measuring out 10 μl specimens using a custom sample cartridge. Renderings drafted in SOLIDWORKS. c, Sample-to-result process for extraction-free sample preparation and plasmonic thermocycling with real-time multispectral fluorescence monitoring. Samples with the buffer are transferred via the sample cartridge to the instrument (step 1). Multispectral fluorescence values against cycle number (step 2) are used to calculate the Ct values for the targets (N1 and N2 primers detect SARS-CoV-2 virus and RP is the human control) and determine the result (step 3). d, Precision of measuring across 11 different cartridges achieved an average dispense volume of 9.2 ± 1.4 μl. e, Classification of positives and negatives from our setup compared with laboratory-based PCR using clinical saliva specimens, and quantitation of Ct values for SARS-CoV-2-positive and SARS-CoV-2-negative specimens. Data are presented as mean ± s.d.

Back to article page